pH6.8buffer ,Mobile phase,Resolution solution,Standard preparation,and Chromatographic system—Prepare as directed in the Assayunder Cefpiramide.

Assay preparation 1 (where it is represented as being in a single-dose container)—Constitute a container of Cefpiramide for Injection in a volume of water,accurately measured,corresponding to the volume of diluent specified in the labeling.Withdraw all of the withdrawable contents,using a suitable hypodermic needle and syringe,and quantitatively dilute with Mobile phaseto obtain a solution containing the equivalent of about 0.25mg of cefpiramide (C25H24N8O7S2)per mL.

Assay preparation 2 (where the label states the quantity of cefpiramide in a given volume of constituted solution)—Constitute a container of Cefpiramide for Injection in a volume of water,accurately measured,equivalent to the volume of diluent specified in the labeling.Quantitatively dilute an accurately measured volume of the constituted solution with water to obtain a solution containing about 0.25mg of cefpiramide (C25H24N8O7S2)per mL.

Procedure— Proceed as directed for Procedurein the Assayunder Cefpiramide.Calculate the quantity,in mg,of cefpiramide (C25H24N8O7S2)withdrawn from the container,or in the portion of constituted solution taken by the formula: in which Lis the labeled quantity,in mg,of cefpiramide (C25H24N8O7S2)in the container,or in the volume of constituted solution taken,Dis the concentration,in mg of cefpiramide per mL,of Assay preparation 1or Assay preparation 2based on the labeled quantity in the container or in the volume of constituted solution taken,respectively,Cis the concentration,in mg per mL,of USP Cefpiramide RSin the Standard preparation,Eis the designated cefpiramide (C25H24N8O7S2)content,in µg per mg,of USP Cefpiramide RS,and rUand rSare the cefpiramide peak responses obtained from the Assay preparationand the Standard preparation,respectively.