A: Dissolve about 200mg of Menadiol Sodium Diphosphate in 10mLof water,add 10mLof 2Nsulfuric acid,10mLof 0.1Nceric sulfate,and 1mLof 30percent hydrogen peroxide previously diluted with 5mLof water,and extract the solution with two 10-mLportions of chloroform.Gently evaporate the clear chloroform solution on a steam bath to dryness,and dry the residue at 80for 1hour:the menadione so obtained melts between 104and 107.

B: To 50mg of the dried residue obtained in Identificationtest Aadd 5mLof water,then add 75mg of sodium bisulfite,and heat on a steam bath,shaking vigorously until the substance is dissolved and the solution is practically colorless.Dilute with water to 50mL,and mix.To 2mLof the solution add 2mLof alcoholic ammonia (prepared by mixing equal volumes of alcohol and ammonium hydroxide),shake,and add 3drops of ethyl cyanoacetate:a deep purplish blue color is produced,and on the addition of 1mLof sodium hydroxide solution (1in 3),it changes to green and then to yellow.

C: To about 20mg contained in a small beaker add 1mLof water,2drops of nitric acid,and 1mLof sulfuric acid,and heat slowly to the evolution of white fumes.Cool,cautiously dilute with water to about 10mL,and filter if not clear.Render the filtrate slightly alkaline to litmus with 6Nammonium hydroxide,then render it acid with nitric acid,and add to the warm solution 3mLof ammonium molybdate TS:a yellow precipitate is formed within a few minutes.