Cholesterol
;)
Cholest-5-en-3-ol,(3b)-.
Cholest-5-en-3b-ol [57-88-5].
»Cholesterol is a steroid alcohol used as an emulsifying agent.
Packaging and storage—
Preserve in well-closed,light-resistant containers.
Solubility in alcohol—
Dissolve 500mg in 50mLof warm alcohol in a stoppered flask or cylinder,and allow to stand at room temperature for 2hours:no deposit or turbidity is formed.
Identification—
A:
To a solution of 10mg in 1mLof chloroform add 1mLof sulfuric acid:the chloroform acquires a blood-red color and the sulfuric acid shows a green fluorescence.
B:
Dissolve about 5mg in 2mLof chloroform,add 1mLof acetic anhydride,and follow with 1drop of sulfuric acid:a pink color is produced,and it rapidly changes to red,then to blue,and finally to a brilliant green.
Acidity—
Dissolve 1.0g in 10mLof ether in a small flask,add 10.0mLof 0.10Nsodium hydroxide,and shake for about 1minute.Heat gently to expel the ether,and then boil for 5minutes.Cool,dilute with 10mLof water,add phenolphthalein TS,and titrate with 0.10Nsulfuric acid until the pink color just disappears,stirring the solution vigorously throughout the titration.Perform a blank determination (see Residual Titrations underTitrimetry á541ñ).The difference between the number of mLof 0.10Nsulfuric acid consumed in the blank and the number of mLconsumed in the test with the Cholesterol is not more than 0.3mL.
Loss on drying á731ñ—
Dry it in vacuum at 60
for 4hours:it loses not more than 0.3%of its weight.
for 4hours:it loses not more than 0.3%of its weight.
Residue on ignition á281ñ:
not more than 0.1%.
Organic volatile impurities,Method IVá467ñ:
meets the requirements.
Auxiliary Information—
Staff Liaison:Daniel K.Bempong,Ph.D.,Scientist
Expert Committee:(EMC)Excipients:Monograph Content
USP28–NF23Page 2989
Phone Number:1-301-816-8143