Medium: 0.05MTris(hydroxymethyl)aminomethane,pH7.6,adjusted,if necessary,with hydrochloric acid to a pHof 7.6;900mL.

Apparatus 2: 75rpm.

Time: 30minutes.

Procedure— Determine the amount of C14H21N3O3Sdissolved from UVabsorbances at the wavelength of maximum absorbance at about 224nm of filtered portions of the solution under test,suitably diluted with Dissolution Medium,in comparison with a Standard solution having a known concentration of USP Tolazamide RSin the same medium.[NOTE—Sonicate the Standard solution until the Reference Standard is dissolved.]

Tolerances— Not less than 70%(Q)of the labeled amount of C14H21N3O3Sis dissolved in 30minutes.

Internal standard preparation ,Mobile phase,and Standard preparation—Prepare as directed in the Assayunder Tolazamide.

Assay preparation— Weigh and finely powder not less than 10Tablets.Weigh accurately a portion of the powder,equivalent to about 300mg of tolazamide,and transfer to a suitable container.Add 100.0mLof Internal standard solutionand about 20glass beads.Securely close the container,and shake vigorously for approximately 30minutes.Centrifuge,and use the clear liquid as the Assay preparation.

Procedure— Proceed as directed for Procedurein the Assayunder Tolazamide.Calculate the quantity,in mg,of C14H21N3O3Sin the portion of Tablets taken by the formula: in which the terms are as defined therein.