Glucose Enzymatic Test Strip
»Glucose Enzymatic Test Strip consists of the enzymes glucose oxidase and horseradish peroxidase,a suitable substrate for the reaction of hydrogen peroxide catalyzed by peroxidase,and other inactive ingredients impregnated and dried on filter paper.When tested in human urine containing known glucose concentrations,it reacts in the specified times to produce colors corresponding to the color chart provided.
Packaging and storage— Preserve in the original container,in a dry place,at controlled room temperature.
Identification— Remove the Strips from the container,and test them as directed in the instructions provided by the manufacturer,first in dextrose solution (1in 50)and then in sucrose solution (1in 50):color develops from the dextrose solution,but not from the sucrose solution.
Calibration—
Glucose standard solutions— Dissolve anhydrous dextrose in separate portions of freshly voided,normal,glucose-free human urine,previously adjusted to a pHof 6.0with dilute formic acid (1in 5),and with sodium hydroxide solution (1in 2),respectively,to obtain separate solutions of the final concentrations corresponding to the color chart calibrations provided.Allow the standard solutions to stand for 1hour prior to use.
Procedure— Remove the Strips from the container,and test each Glucose standard solutionas directed in the instructions provided by the manufacturer:the colors formed on each of the Strips during the specified times match the colors on the color chart provided.
Auxiliary Information— Staff Liaison:Radhakrishna S Tirumalai,Scientist
Expert Committee:(GTB)General Toxicology and Biocompatibility
USP28–NF23Page 909
Phone Number:1-301-816-8339