A: Infrared Absorption á197Kñ.

B: Dissolve about 100mg in 50mLof 1Nsodium hydroxide,and reflux for 15minutes.Cool,and add 3mLof the refluxed solution to 25mLof saturated sodium bicarbonate solution.Add,with mixing,4mLof N,N-dimethyl-p-phenylenediamine dihydrochloride solution (1.5in 10,000)and 2mLof sodium hypochlorite solution (1.5in 20,000):a violet-blue color is produced.

C: Dissolve about 50mg in 20mLof water,add 10mLof a 1in 50solution of ammonium reineckate in methanol,and allow to stand for 30minutes with occasional swirling:a pink reineckate of demecarium forms,and it melts between 131and 136,with decomposition.

D: Asolution of it responds to the tests for Bromide á191ñ.

Control solution— Dissolve 100mg of m-dimethylaminophenol in 10mLof alcohol in a 1000-mLvolumetric flask,dilute with water to volume,and mix.Pipet 1mLof this solution into a 500-mLvolumetric flask,dilute with water to volume,and mix.

Test solution— Transfer 100mg of Demecarium Bromide to a 100-mLvolumetric flask,add water to volume,and mix.

Procedure— Pipet 25mLof the Test solutioninto a glass-stoppered,50-mLcentrifuge tube,and pipet 25mLof the Control solutioninto a second,similar tube.To each tube add 3mLof pH7.0phosphate buffer (see under Solutionsin the section Reagents,Indicators,and Solutions),1mLof N,N-dimethyl-p-phenylenediamine dihydrochloride solution (1.5in 10,000),5mLof isobutyl alcohol,and 1mLof sodium hypochlorite solution (1.5in 20,000).Insert the stoppers in the tubes,shake the mixtures for 5minutes,and centrifuge:any blue color produced in the upper layer obtained from the Test solutionis not more intense than that obtained from the Control solution.