Cupric Chloride
CuCl2·2H2O 170.48

Copper chloride (CuCl2)dihydrate.
Copper(2+)chloride dihydrate [10125-13-0].

Anhydrous 134.45 [7447-39-4].
»Cupric Chloride contains not less than 99.0percent and not more than 100.5percent of CuCl2,calculated on the dried basis.
Packaging and storage— Preserve in tight containers.Store at 25,excursions permitted between 15and 30.
Identification— Asolution (1in 20)responds to the tests for Chloride á191ñand for Copper á191ñ.
Loss on drying á731ñ— Dry it at 105for 16hours:it loses between 20.9%and 21.4%of its weight.
Insoluble matter— Transfer 10g to a 250-mLbeaker,add 100mLof water and 2mLof hydrochloric acid,cover the beaker,and heat to boiling.Digest the hot solution on a steam bath for 1hour,and filter through a tared,fine-porosity filtering crucible.Rinse the beaker with hot water,passing the rinsings through the filter,and finally wash the filter with additional hot water.[NOTE—Retain the combined filtrate and washings for the test for Sulfate.]Dry the filter at 105:the residue weighs not more than 1.0mg (0.01%).
Sulfate— Heat to boiling the combined filtrate and washings retained from the test for Insoluble matter,add 10mLof barium chloride TS,digest for 2hours on a steam bath,and allow to stand overnight.Filter the solution through a tared,medium-porosity porcelain filtering crucible,and wash the residue with two 10-mLportions of hot water.Ignite at 800±25to constant weight:the weight of the residue,corrected for the weight obtained in a blank test,does not exceed 1.2mg (0.005%).
Limit of sodium—
Stock solution— Transfer 10.0g of Cupric Chloride to a 100-mLvolumetric flask,add water,and swirl to dissolve.Add 5mLof nitric acid,dilute with water to volume,and mix.This solution contains 0.1g of cupric chloride per mL.
Test solutions— To three 25-mLvolumetric flasks add a volume of Stock solutionequivalent to the weight of test specimen given in the accompanying table.To two of the flasks add the amounts of reference analyte ion specified in the accompanying table.Add 2mLof potassium chloride solution (1in 20)to each flask,dilute with water to volume,and mix.
Procedure— Using atomic absorption spectrophotometry (see Spectrophotometry and Light-Scattering á851ñ),analyze the Test solutionsby the method of standard addition analysis according to the accompanying table.The limit is 0.02%.
Limit of potassium—
Stock solution— Use the Stock solutionprepared in the test for Limit of sodium.
Test solutions— To three 25-mLvolumetric flasks add a volume of Stock solutionequivalent to the weight of test specimen given in the accompanying table.To two of the flasks add the amounts of reference analyte ion specified in the accompanying table,dilute with water to volume,and mix.
Procedure— Using atomic absorption spectrophotometry (see Spectrophotometry and Light-Scattering á851ñ),analyze the Test solutionsby the method of standard addition analysis according to the accompanying table.The limit is 0.01%.
Limit of calcium—
Stock solution— Use the Stock solutionprepared in the test for Limit of sodium.
Test solutions— To three 25-mLvolumetric flasks add a volume of Stock solutionequivalent to the weight of test specimen given in the accompanying table.To two of the flasks add the amounts of reference analyte ion specified in the accompanying table,dilute with water to volume,and mix.
Procedure— Using atomic absorption spectrophotometry (see Spectrophotometry and Light-Scattering á851ñ),analyze the Test solutionsby the method of standard addition analysis according to the accompanying table.The limit is 0.005%.
Limit of iron—
Stock solution— Use the Stock solutionprepared in the test for Limit of sodium.
Test solutions— To three 25-mLvolumetric flasks add a volume of Stock solutionequivalent to the weight of test specimen given in the accompanying table.To two of the flasks add the amounts of reference analyte ion specified in the accompanying table,dilute with water to volume,and mix.
Procedure— Using atomic absorption spectrophotometry (see Spectrophotometry and Light-Scattering á851ñ),analyze the Test solutionsby the method of standard addition analysis according to the accompanying table.The limit is 0.005%.
Limit of nickel—
Stock solution— Use the Stock solutionprepared in the test for Limit of sodium.
Test solutions— To three 25-mLvolumetric flasks add a volume of Stock solutionequivalent to the weight of test specimen given in the accompanying table.To two of the flasks add the amounts of reference analyte ion specified in the accompanying table,dilute with water to volume,and mix.
Procedure— Using atomic absorption spectrophotometry (see Spectrophotometry and Light-Scattering á851ñ),analyze the Test solutionsby the method of standard addition analysis according to the accompanying table.The limit is 0.01%.
Organic volatile impurities,Method IVá467ñ: meets the requirements.
Solvent— Use dimethyl sulfoxide.
Assay— Transfer about 400mg of Cupric Chloride,accurately weighed,to a beaker,and dissolve in 50mLof water.Add 4mLof acetic acid and 3g of potassium iodide,mix,and titrate the liberated iodine with 0.1Nsodium thiosulfate VS,adding about 2g of potassium thiocyanate and 3mLof starch TSas the endpoint is approached.Each mLof 0.1Nsodium thiosulfate is equivalent to 13.45mg of CuCl2.
Limit test Wavelength
(nm)		 Test specimen
weight (g)		 Reference ion
added (mg)		 Flame type Background
correction
Sodium 589.0 0.1 0.01/0.02 air-acetylene No
Potassium 766.5 0.1 0.01/0.02 air-acetylene No
Calcium 422.7 2.0 0.05/0.10 air-acetylene No
Iron 248.3 2.0 0.05/0.10 air-acetylene Yes
Nickel 232.0 2.0 0.10/0.20 air-acetylene No
Auxiliary Information— Staff Liaison:Behnam Davani,Ph.D.,MBA,Senior Scientist
Expert Committee:(PA7)Pharmaceutical Analysis 7
USP28–NF23Page 552
Pharmacopeial Forum:Volume No.29(6)Page 1864
Phone Number:1-301-816-8394