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CHROMATOGRAPHIC REAGENTS
The following list of packings (L),phases (G),and supports (S)is intended to be a convenient reference for the chromatographer.[NOTE—Particle sizes given in this listing are those generally provided.Where other,usually finer,sizes are required,the individual monograph specifies the desired particle size.Within any category of packings or phases listed below,there may be a wide range of columns available.Where it is necessary to define more specifically the chromatographic conditions,the individual monograph so indicates.]
Packings
L1—Octadecyl silane chemically bonded to porous silica or ceramic micro-particles,3to 10µm in diameter.
L2—Octadecyl silane chemically bonded to silica gel of a controlled surface porosity that has been bonded to a solid spherical core,30to 50µm in diameter.
L3—Porous silica particles,5to 10µm in diameter.
L4—Silica gel of controlled surface porosity bonded to a solid spherical core,30to 50µm in diameter.
L5—Alumina of controlled surface porosity bonded to a solid spherical core,30to 50µm in diameter.
L6—Strong cation-exchange packing–sulfonated fluorocarbon polymer coated on a solid spherical core,30to 50µm in diameter.
L7—Octylsilane chemically bonded to totally porous silica particles,3to 10µm in diameter.
L8—An essentially monomolecular layer of aminopropylsilane chemically bonded to totally porous silica gel support,10µm in diameter.
L9—10-µm irregular or spherical,totally porous silica gel having a chemically bonded,strongly acidic cation-exchange coating.
L10—Nitrile groups chemically bonded to porous silica particles,3to 10µm in diameter.
L11—Phenyl groups chemically bonded to porous silica particles,5to 10µm in diameter.
L12—Astrong anion-exchange packing made by chemically bonding a quaternary amine to a solid silica spherical core,30to 50µm in diameter.
L13—Trimethylsilane chemically bonded to porous silica particles,3to 10µm in diameter.
L14—Silica gel 10µm in diameter having a chemically bonded,strongly basic quaternary ammonium anion-exchange coating.
L15—Hexylsilane chemically bonded to totally porous silica particles,3to 10µm in diameter.
L16—Dimethylsilane chemically bonded to porous silica particles,5to 10µm in diameter.
L17—Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the hydrogen form,7to 11µm in diameter.
L18—Amino and cyano groups chemically bonded to porous silica particles,3to 10µm in diameter.
L19—Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the calcium form,about 9µm in diameter.
L20—Dihydroxypropane groups chemically bonded to porous silica particles,5to 10µm in diameter.
L21—Arigid,spherical styrene-divinylbenzene copolymer,5to 10µm in diameter.
L22—Acation-exchange resin made of porous polystyrene gel with sulfonic acid groups,about 10µm in size.
L23—An anion-exchange resin made of porous polymethacrylate or polyacrylate gel with quaternary ammonium groups,about 10µm in size.
L24—Asemi-rigid hydrophilic gel consisting of vinyl polymers with numerous hydroxyl groups on the matrix surface,32to 63µm in diameter.5
L25—Packing having the capacity to separate compounds with a molecular weight range from 100–5000(as determined by polyethylene oxide),applied to neutral,anionic,and cationic water-soluble polymers.Apolymethacrylate resin base,cross-linked with polyhydroxylated ether (surface contained some residual carboxyl functional groups)was found suitable.
L26—Butyl silane chemically bonded to totally porous silica particles,5to 10µm in diameter.
L27—Porous silica particles,30to 50µm in diameter.
L28—Amultifunctional support,which consists of a high purity,100Å,spherical silica substrate that has been bonded with anionic exchanger,amine functionality in addition to a conventional reversed phase C8functionality.
L29—Gamma alumina,reverse-phase,low carbon percentage by weight,alumina-based polybutadiene spherical particles,5µm in diameter with a pore volume of 80Å.
L30—Ethyl silane chemically bonded to totally porous silica particles,3to 10µm in diameter.
L31—Astrong anion-exchange resin-quaternary amine bonded on latex particles attached to a core of 8.5-µm macroporous particles having a pore size of 2000Åand consisting of ethylvinylbenzene cross-linked with 55%divinylbenzene.
L32—Achiral ligand-exchange packing–L-proline copper complex covalently bonded to irregularly shaped silica particles,5to 10µm in diameter.
L33—Packing having the capacity to separate dextrans by molecular size over a range of 4,000to 500,000Da.It is spherical,silica-based,and processed to provide pHstability.6
L34—Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the lead form,about 9µm in diameter.
L35—Azirconium-stabilized spherical silica packing with a hydrophilic (diol-type)molecular monolayer bonded phase having a pore size of 150Å.
L36—A3,5-dinitrobenzoyl derivative of L-phenylglycine covalently bonded to 5-µm aminopropyl silica.
L37—Packing having the capacity to separate proteins by molecular size over a range of 2,000to 40,000Da.It is a polymethacrylate gel.
L38—Amethacrylate-based size-exclusion packing for water-soluble samples.
L39—Ahydrophilic polyhydroxymethacrylate gel of totally porous spherical resin.
L40—Cellulose tris-3,5-dimethylphenylcarbamate coated porous silica particles,5to 20µm in diameter.
L41—Immobilized a1-acid glycoprotein on spherical silica particles,5µm in diameter.
L42—Octylsilane and octadecylsilane groups chemically bonded to porous silica particles,5µm in diameter.
L43—Pentafluorophenyl groups chemically bonded to silica particles by a propyl spacer,5to 10µm in diameter.
L44—Amultifunctional support,which consists of a high purity,60Å,spherical silica substrate that has been bonded with a cationic exchanger,sulfonic acid functionality in addition to a conventional reversed phase C8functionality.
L45—Beta cyclodextrin bonded to porous silica particles,5to 10µm in diameter.
L46—Polystyrene/divinylbenzene substrate agglomerated with quaternary amine functionalized latex beads,10µm in diameter.
L47—High-capacity anion-exchange microporous substrate,fully functionalized with trimethlyamine groups,8µm in diameter.7
L48—Sulfonated,cross-linked polystyrene with an outer layer of submicron,porous,anion-exchange microbeads,15µm in diameter.
L49—Areversed-phase packing made by coating a thin layer of polybutadiene onto spherical porous zirconia particles,3to 10µm in diameter.8
L50—Multifunction resin with reversed-phase retention and strong anion-exchange functionalities.The resin consists of ethylvinylbenzene,55%cross-linked with divinylbenzene copolymer,3to 15µm in diameter,and a surface area not less than 350m2per g.Substrate is coated with quaternary ammonium functionalized latex particles consisting of styrene cross-linked with divinylbenzene.9
L51—Amylose tris-3,5-dimethylphenylcarbamate-coated,porous,spherical,silica particles,5to 10µm in diameter.10
L52—Astrong cation exchange resin made of porous silica with sulfopropyl groups,5to 10µm in diameter.11
L53—Weak cation-exchange resin consisting of ethylvinylbenzene,55%cross-linked with divinylbenzene copolymer,3to 15µm diameter.Substrate is surface grafted with carboxylic acid and/or phosphoric acid functionalized monomers.Capacity not less than 500µEq/column.12
L54—Asize exclusion medium made of covalent bonding of dextran to highly cross-linked porous agarose beads,about 13µm in diameter.13
L55—Astrong cation-exchange resin made of porous silica coated with polybutadiene–maleic acid copolymer,about 5µm in diameter.14
L56—Isopropyl silane chemically bonded to totally porous silica particles,3to 10µm in diameter.15
L57—Achiral-recognition protein,ovomucoid,chemically bonded to silica particles,about 5µm in diameter,with a pore size of 120Å.
L58—Strong cation-exchange resin consisting of sulfonated cross-linked styrene-divinylbenzene copolymer in the sodium form,about 7to 11µm in diameter.16
L59—Packing having the capacity to separate proteins by molecular weight over the range of 10to 500kDa.It is spherical (10µm),silica-based,and processed to provide hydrophilic characteristics and pHstability.17USP28
L60—Spherical,porous silica gel,3or 5µm in diameter,the surface of which has been covalently modified with palmitamidopropyl groups and endcapped with acetamidopropyl groups to a ligand density of about 6µmoles per m2.18USP28
Phases
G1—Dimethylpolysiloxane oil.
G2—Dimethylpolysiloxane gum.
G3—50%Phenyl-50%methylpolysiloxane.
G4—Diethylene glycol succinate polyester.
G5—3-Cyanopropylpolysiloxane.
G6—Trifluoropropylmethylpolysiloxane.
G7—50%3-Cyanopropyl-50%phenylmethylsilicone.
G8—80%Bis(3-cyanopropyl)-20%3-cyanopropylphenylpolysiloxane (percentages refer to molar substitution).
G9—Methylvinylpolysiloxane.
G10—Polyamide formed by reacting a C36dicarboxylic acid with 1,3-di-4-piperidylpropane and piperidine in the respective mole ratios of 1.00:0.90:0.20.
G11—Bis(2-ethylhexyl)sebacate polyester.
G12—Phenyldiethanolamine succinate polyester.
G13—Sorbitol.
G14—Polyethylene glycol (av.mol.wt.of 950to 1050).
G15—Polyethylene glycol (av.mol.wt.of 3000to 3700).
G16—Polyethylene glycol compound (av.mol.wt.about 15,000).Ahigh molecular weight compound of polyethylene glycol with a diepoxide linker.Available commercially as Polyethylene Glycol Compound 20M,or as Carbowax 20M,from suppliers of chromatographic reagents.
G17—75%Phenyl-25%methylpolysiloxane.
G18—Polyalkylene glycol.
G19—25%Phenyl-25%cyanopropyl-50%methylsilicone.
G20—Polyethylene glycol (av.mol.wt.of 380to 420).
G21—Neopentyl glycol succinate.
G22—Bis(2-ethylhexyl)phthalate.
G23—Polyethylene glycol adipate.
G24—Diisodecyl phthalate.
G25—Polyethylene glycol compound TPA.Ahigh molecular weight compound of a polyethylene glycol and a diepoxide that is esterified with terephthalic acid.Available commercially as Carbowax 20M-TPAfrom suppliers of chromatographic reagents.
G26—25%2-Cyanoethyl-75%methylpolysiloxane.
G27—5%Phenyl-95%methylpolysiloxane.
G28—25%Phenyl-75%methylpolysiloxane.
G29—3,3¢-Thiodipropionitrile.
G30—Tetraethylene glycol dimethyl ether.
G31—Nonylphenoxypoly(ethyleneoxy)ethanol (av.ethyleneoxy chain length is 30);Nonoxynol 30.
G32—20%Phenylmethyl-80%dimethylpolysiloxane.
G33—20%Carborane-80%methylsilicone.
G34—Diethylene glycol succinate polyester stabilized with phosphoric acid.
G35—Ahigh molecular weight compound of a polyethylene glycol and a diepoxide that is esterified with nitroterephthalic acid.
G36—1%Vinyl-5%phenylmethylpolysiloxane.
G37—Polyimide.
G38—Phase G1containing a small percentage of a tailing inhibitor.19
G39—Polyethylene glycol (av.mol.wt.about 1500).
G40—Ethylene glycol adipate.
G41—Phenylmethyldimethylsilicone (10%phenyl-substituted).
G42—35%phenyl-65%dimethylpolysiloxane (percentages refer to molar substitution).
G43—6%cyanopropylphenyl-94%dimethylpolysiloxane (percentages refer to molar substitution).
G44—2%low molecular weight petrolatum hydrocarbon grease and 1%solution of potassium hydroxide.
G45—Divinylbenzene-ethylene glycol-dimethylacrylate.
G46—14%Cyanopropylphenyl-86%methylpolysiloxane.
G47—Polyethylene glycol (av.mol.wt.of about 8000).
G48—Highly polar,partially cross-linked cyanopolysiloxane.
G49—Proprietary derivatized phenyl groups on a polysiloxane backbone.20
Supports
NOTE—Unless otherwise specified,mesh sizes of 80to 100or,alternatively,100to 120are intended.
S1A—Siliceous earth for gas chromatography has been flux-calcined by mixing diatomite with Na2CO3flux and calcining above 900.The siliceous earth is acid-washed,then water-washed until neutral,but not base-washed.The siliceous earth may be silanized by treating with an agent such as dimethyldichlorosilane21to mask surface silanol groups.
S1AB—The siliceous earth as described above is both acid-and base-washed.21
S1C—Asupport prepared from crushed firebrick and calcined or burned with a clay binder above 900with subsequent acid-wash.It may be silanized.
S1NS—The siliceous earth is untreated.
S2—Styrene-divinylbenzene copolymer having a nominal surface area of less than 50m2per g and an average pore diameter of 0.3to 0.4µm.
S3—Copolymer of ethylvinylbenzene and divinylbenzene having a nominal surface area of 500to 600m2per g and an average pore diameter of 0.0075µm.
S4—Styrene-divinylbenzene copolymer with aromatic –Oand –Ngroups,having a nominal surface area of 400to 600m2per g and an average pore diameter of 0.0076µm.
S5—40-to 60-mesh,high-molecular weight tetrafluorethylene polymer.
S6—Styrene-divinylbenzene copolymer having a nominal surface area of 250to 350m2per g and an average pore diameter of 0.0091µm.
S7—Graphitized carbon having a nominal surface area of 12m2per g.
S8—Copolymer of 4-vinyl-pyridine and styrene-divinylbenzene.
S9—Aporous polymer based on 2,6-diphenyl-p-phenylene oxide.
S10—Ahighly polar cross-linked copolymer of acrylonitrite and divinylbenzene.
S11—Graphitized carbon having a nominal surface area of 100m2per g modified with small amounts of petrolatum and polyethylene glycol compound.22
S12—Graphitized carbon having a nominal surface area of 100m2per g.